Which color do Gram-negative bacteria appear after the Gram stain process?

Prepare for your Jones and Bartlett Learning Module 3 exam with comprehensive study materials. Utilize practice questions, flashcards, and detailed explanations to enhance your understanding and readiness. Ace your test with confidence!

Multiple Choice

Which color do Gram-negative bacteria appear after the Gram stain process?

Explanation:
Gram-negative bacteria appear pink after the Gram stain because their cell wall structure causes them to lose the initial crystal violet-iodine complex during the decolorization step and then take up the counterstain. The thin peptidoglycan layer and the presence of an outer membrane allow the alcohol to wash away the purple color. When the counterstain, typically safranin, is applied, these cells pick up the pink/red color, visible under the microscope. In contrast, Gram-positive bacteria retain the purple stain due to their thicker peptidoglycan layer.

Gram-negative bacteria appear pink after the Gram stain because their cell wall structure causes them to lose the initial crystal violet-iodine complex during the decolorization step and then take up the counterstain. The thin peptidoglycan layer and the presence of an outer membrane allow the alcohol to wash away the purple color. When the counterstain, typically safranin, is applied, these cells pick up the pink/red color, visible under the microscope. In contrast, Gram-positive bacteria retain the purple stain due to their thicker peptidoglycan layer.

Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy